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5 Agar 7 Final pH 7
G-418 can by inactivated by NPTII or 3APH
The antibiotic binds reversible to the 30S subunit of the ribosome
To avoid errors due to this reserve of nitrogen it is advised to make two serial transfers in the medium
Potassium phosphate is present in Terrific broth buffered to balance the pH and subsequently prevent cell death due to a drop in pH
ME Agar Size:250g 5 Agar 7 Final pHFormula g l Malt Extract 30 Agar 17 Adenine 0. 05 L Histidine 0. 05 L Leucine 0. 05 Uracil 0. 05 Total 47. 20 Suspend 47. 2 gram powdered medium in 1 litre distilled water. Store dry at room temperature.
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