Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:1000g Adjust to pH 6

Adjust to pH 6

phenylalanine and glycine

5 Thin Layer Chromatogram One Spot Potency >720 µ/mg Store at 2 - 8°C

35 mM) of KCl is added

Tryptone 12 Yeast Extract 24 KH2PO4 2

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine Size:1000g Adjust to pH 6Thiamine HCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of thiamine or to replace this vitamin. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room