Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Addition of magnesium and calcium

Addition of magnesium and calcium increases the growth of the recombinant cells

15 g SP supplements ( Ade

2 Manganese Sulfate 0

5 Thin Layer Chromatogram One Spot Potency >720 µ/mg Water Purity Used Resistivity at 25°C

These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium Size:500g Addition of magnesium and calciumSodium chloride, NaCl is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Sodium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room